Part:BBa_K2665020:Design

SED1 secretion signal-FgBF3-ddFLN4-FLAG tag-ybbr tag-Stalk Region-SED1 anchoring domain

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 468
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2809
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 624
  Illegal SapI site found at 944
  Illegal SapI site found at 2746
  Illegal SapI.rc site found at 126
  

Design Notes

a

Source

from a paper(reference)